COMPUTER-ORIENTED ANALYSIS OF HUMAN CHROMOSOMES IV. DEOXYRIBONUCLEIC ACID-BASED CENTROMERIC INDEX
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چکیده
منابع مشابه
Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes.
Fluorescence of the dye 33258 Hoechst bound to chromosomes is partially quenched by the introduction of BrdU into chromosomal DNA. This finding has allowed microfluorometric detection of DNA synthesis in human metaphase chromosomes. Incorporation, shortly before cell harvest, of a pulse of thymidine into chromosomes otherwise substituted with BrdU results in sharply defined foci of bright 33258...
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The properties of Azotobacter vinelandii deoxyribonucleic acid (DNA) and folded chromosomes were studied and compared to those of Escherichia coli as a standard. Based on melting temperature and buoyant density measurements, the guanosine + cytosine content of purified A. vinelandii DNA was 65%, whereas that of E. coli DNA was 50%. The results of renaturation studies showed that the unique DNA ...
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Measurements of the quasistatic and frequency dependent electrical conductivity below 1 MHz were carried out on wet-spun, macroscopically oriented, calf thymus deoxyribonucleic (DNA) and umbilical cord hyaluronic acid (HA) bulk samples. The frequency dependence of the electrical conductivity in the frequency range of approximately 10(-3) - 10(6) Hz of both materials is surprisingly rather simil...
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Although the synthesis of DNA1 by an enzyme purified from Escherichia coli requires four deoxynucleoside triphosphates (dAPPP, dCPPP, dGPPP, TPPP) in addition to Mg++ and DNA,2* a nevertheless a single deoxynucleoside triphosphate can be incorporated into DNA to an extent about one-thousandth of that for the complete reaction. a The number of the single . deoxynucleotides incorporated is of the...
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Deoxyribonucleic acid (DNA)-DNA hybridization on nitrocellulose filters can be used to assay for replication origin DNA from Escherichia coli if the DNA attached to the filters is enriched for the replication origin sequences. Such DNA can be readily isolated from very rapidly growing cells. When low amounts of this DNA were attached to filters, radioactively labeled DNA from the replication or...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1974
ISSN: 0022-1554,1551-5044
DOI: 10.1177/22.7.554